Very first thing I did was a little math. I knew I didn’t want to make more than 10ml worth of E-Juice and I didn’t want the flavorings to exceed 10%; which meant I need 9ml worth of diluent or my base. Now I know a lot of vendors deal with pure nicotine and mix it themselves to cut down on costs. But this is also very dangerous as I was at home doing this in my dining room table.

According to the of the grant proposal, the research question that the lab team is proposing to investigate is as follows: "The goal of this application is to determine the effects of Third Hand Smoke on wound healing. ... It is known that first-hand smoke and secondhand smoke (SHS) inhibit wound healing but nothing is known about the effects of third-hand smoke (THS) on wound healing. ... To address this problem, we will use mice, expose them for 6 months to THS present in their cages and, at the end of the exposure period, we will perform wounds on their backs and study the time course of healing by examining not only the wound areas but also the properties of the tissues."

Disclosure: I have not received any funding or compensation from the tobacco, electronic cigarette, or pharmaceutical industries. However, I am seeking funding from several electronic cigarette companies to conduct a behavioral study on the effects of electronic cigarettes on smoking behavior.

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